Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
NEW ENGLAND BIOGROUP LUNA Acridine Orange Propidium Iodide, sterile filtered, 2 x .5mL. For use with LUNA-FL and LUNA-FX7 automated cell counters.
LUNA Acridine Orange Propidium Iodide, sterile filtered, 2 x .5mL. For use with LUNA-FL and LUNA-FX7 automated cell counters.
New England Biolabs, Inc. Luna® Universal One-Step RT-qPCR Kit – 1000 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Norgen Biotek Corp 2X One-Step Rt-Pcr Mas 100Rxns
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1RT Source: An E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Alkali Scientific Radiant Green Lo-ROX qPCR Kit, 5,000 Reactions, (50 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ qPCR Genotyping Mix, No-ROX, Suitable for Dual-labeled Taqman™ and Other Probes, 100 Reactions, (1 X 1mL)
Using the latest advancements in polymerase technology and advanced buffer chemistry Radiant™ Genotyping Mix No-Rox offers market leading performance with minimal optimization. Radiant™ Genotyping Mix No-Rox is a kit designed for use in dual-labeled probe based genotyping assays including Taqman, Molecular Beacon and Scorpion probe genotyping. Radiant™ GenotypingMix is fully compatible with ABI TaqMan Pre-Designed SNP Genotyping assays. Radiant™ Genotyping Mix uses proprietary small molecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity. Highthroughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.
Cytek Biosciences Inc CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ..... Cat. No. 31-5202-0100R ..... Size 100 rxns
Alkali Scientific Radiant™ HRM Mix, High resolution melt - SNP detection, 500 Reactions, (5 x 1mL)
Radiant™HRM Mix produces ultra-sensitive melt profiles allowing accurate discrimination subtle sequence variations including class I to class IV mutations and CpG methylation differences. Fast and accurate detection of subtle sequence differences Ultra-sensitive fluorescence profiles for clear characterisation of samples Third generation saturating dye SyGreen 2 Antibody-mediated hot start technology for improved sensitivity Compatible with all real-time instruments with HRM capability
Cytek Biosciences Inc FastPROBE™ qPCR Lo-ROX Master Mix
FastPROBE™ qPCR Lo-ROX Master Mix ..... Cat. No. 31-5111-0100R ..... Size 100 rxns
Cytek Biosciences Inc CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit
CYBRFast™ 1-Step RT-qPCR Hi-ROX Kit ..... Cat. No. 31-5202-0300R ..... Size 300 rxns
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR Kit (No ROX) – 2500 reactions
In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed, ROX can be added.)
Zymo Research Corporation Quick-cfRNA Digestion Buffer, 30 ml
A component for Quick-cfRNA Serum & Plasma Kit
Ops Diagnostics 200 micron Low Binding Zirconium Beads, 250 gm
Low Binding Beads are chemically treated so they bind less solutes liberated from homogenized samples. Untreated silica and zirconium beads have surface chemistries that bind DNA, RNA, and proteins upon disruption of cells. Both enzyme and PCR-based assays have lower sensitivity when analytes are liberated using untreated beads. Low Binding Beads yield a greater concentration of solutes and provide greater range and linearity, especially in real-time PCR assays.